Today I plated millions of bacteria!!!
Three strains, KW20, recJ-, exoI- had previously been made competent in MIV starvation medium. I gave them some MAP7 DNA to take up, diluted them, then plated them on novobiocin plates to select for transformants.
The second thing I did today was make DNA from dprA::kan cells (grown last night). This required adding chemicals, extracting twice, adding more chemicals, then scooping out the glump (read: gluey clump) of chromosomal DNA. Most exciting part was when I had to make phenol/chloroform (one of the added chemicals). Looking into the shelves underneath the fumehood was like looking into ... a prison. For chemicals.
I then took this DNA, gave it to competent KW20 cells, let them express the gene, then plated them. Hopefully, results will show tomorrow.