Wednesday, May 6, 2009

Tomorrow will be a happenin' day.  The plan is to test competences of all the strains I have accumulated so far (recJ-, exoI-, and wildtype KW20) with MAP7 DNA and select for novobiocin.  Since the recJ- and exoI- are missing nucleases (enzymes that chop up DNA), they should have similar/same transformation frequencies as the wildtype.  dprA- will be tested as well... if only I could find the DNA in the freezer.  0_0

I should've blogged while I was in the lab... now I can't remember squat.

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