Tomorrow will be a happenin' day. The plan is to test competences of all the strains I have accumulated so far (recJ-, exoI-, and wildtype KW20) with MAP7 DNA and select for novobiocin. Since the recJ- and exoI- are missing nucleases (enzymes that chop up DNA), they should have similar/same transformation frequencies as the wildtype. dprA- will be tested as well... if only I could find the DNA in the freezer. 0_0
I should've blogged while I was in the lab... now I can't remember squat.