Friday, June 12, 2009

I grew competent cells all day. They took forever (i.e. 5-6 hours) to get to an OD600 of ~0.2. Glehhhh. I'm still waiting for them to finish their "growing for 100 minutes in MIV solution" stage. Hopefully more exciting things to come next week. Stay tuned.

1 comment:

Rosie Redfield said...

Why did they take so long to reach the right density? Can you tell whether they were growing slowly or whether you just started with few viable cells? And where did the cells you started with come from?