You'd be surprised at how time flies when you're watching ice melt (and preventing it from spreading on the floor).
I spent most of today defrosting our -20C freezer, and the to-do list for this week includes:
1. Make KW20, dprA-, RecJ-, ExoI-, dprARecJ--, and dprAExoI-- cells MIV-competent in order to...
2. Transform these strains with MAP7 DNA to determine how well (or how badly) they take up DNA.
3. Run a successful PCR with wildtype DNA (probably MAP7) and RecJ, ExoI, and crp primers in order to practise PCR-ing and actually get it to work.
I'll be doing steps 1 and 2 in sets: [KW20, RecJ-, dprA-, RecJdprA--] is one and [KW20, ExoI-, dprA-, and ExoIdprA--] is another. This is so that comparisons can be made between strains without having to worry too much about differences in biology that occur due to experimentation on different days.