Planning to make some more MIV-competent cells ASAP, as I've used them all in transformations. Actually, I did a transformation today. It's a replicate with KW20, ExoI-, dprA-, and ExoI-dprA- taking up MAP7 DNA and un/successfully expressing novobiocin resistance.
Also, we got new Taq today and I ran a PCR right alongside SS. If nothing shows up, woe is me. If I get bands, I'll be so relieved I might consider supporting Radio Paradise. (JK; that only came to mind because I hear it many, many times daily.) I'll put the samples in the fridge and run them on a gel tomorrow.