Thursday, August 6, 2009

Did PCR to amplify comM gene from KW20 x-somal DNA, then ran a gel to check the product. There were two bands, not one, so I increased the annealing temperature (from 50 to 55 degrees Celsius) and am running another PCR.

The plates from yesterday's dprA- survival curve were ready to count, but sadly, all of the plain plates got contaminated (turns out it was the BHI!!! gah!!). The kan plates were fine, and I did count them, but since the growth of dprA- Hin cells could have been affected by the contamination, I don't trust the numbers very much.

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