Monday, August 10, 2009

Today I ligated Hin's comM gene into pTOPO cloning vector. Last week, I amplified comM through PCR and purified it (with a kit hehehehe). The ligation was very very quick; probably didn't even take 30 minutes! I also transformed this cloning vector into DH5-alpha (does blogger speak Greek?), a strain of E.coli, then plated the cells on LB again containing ampicillin and X-gal. It was ... funny working with something other than Hin (E.coli even smells different), but it's cool to actually do something that I've studied in class! I remember learning about X-gal and the colonies appearing white if the cloning vectors have ligated with a gene (blue if they haven't).

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